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放射線照射時胎內白鼠의 指趾畸形成立過程에 關한 實驗的硏究
A Study of Morphogenesis of Digital Malformation on Rat Embryo By X-Irradiation

치과방사선 1981년 11권 1호 p.33 ~ 40

소속 상세정보

김재덕(金在德)/Jai Dhuck Kim

KMID : 0378219810110010033

Abstract

著者는 受胎後 11½日째 되는 姙娠白鼠에 X-線을 照射해서 그 胎兒에 指趾畸形을 成立시
키고 그 成立過程에 對해 H-E 染色과 Nile blue의 超生體染色法에 의해 觀察한 結果 다음
과 같은 結論을 얻었다.
1. 胎齡 11½日에 X-線照射를 行하여 白鼠胎兒의 欠指趾, 多趾, 癒合指趾 및 血腫趾等의
崎形을 誘導하였다. 發生樣狀은 欠指趾에서는 線量果를 나타내었으나 癒合趾 및 多趾에서
는 線量效果를 나타내지 않았다.
2. X-線照射에 依해 肢牙의 細胞壞死는 顯著하게 나타나나 照射後 48時間에서는 暄芽周
邊에만 壓排되었고 .照射後 72時間에는 消失되었다. 指放線은 48時間에 이르러 그 欠如, 不
整等의 狀態가 明瞭히 觀察되었으며 照射後 72時閒頃에는 對照群과 같은 程度의 生理的細
胞壞死가 指間함몰부에서 觀察되었다. 癒含指趾로 指間함몰을 나타내지 않는 것에서는 生理
的細胞壞死를 認定할 수 없었다.
3. X-線照射에 依해 誘導되어진 欠指趾는 指原基의 壞死가 直接的인 成立要因으로 惹起
된다고 생각되나 多指, 癒合指指는 X-線障碍의 恢復過程에 主原因이 있다고 思料된다.
#초록#
The author studied on the effects of x-irradiation to the development of digital
malformation in gestation rats. The time-matings occurred between 6 p.m. and 8 a.m.
and females with copulation plugs at 8 a.m. were isolated and properly marked for
evidence of copulation. The lower abdomen of mothers were exposed to x-irradiation on
the 11½th day of gestation, the critical period developing digital malformation,
respectively 100, 150, 200, 250, 300 and 350 rads. At 18½th day of post-conception total
50 pregnant females were dissected and the incidence of digital malformations were
obtained. Rat embryos on the 12, 13, 14, 15, 16th day of gestation irradiated by 250 rads
were examined for morphogenesis of digital malformation. Digital radiating lines were
examined in water and histologically by H-E stain. Supra vital stain samples by
Nile-blue sulfate in 37℃ normal saline were prepared for the observation of cell necrosis
regions and morphogenesis of digits. The results obtained were as follows ;
1. By x-irradiation on 11th day of gestation, digital malformations of Ectrodactylia,
Syndactylia, Polydactylia and Hematodactylia were developed. Ectrodactylia showed the
effective relationship to the amount of irradiation, however Syndactylia and Polydactylia
did not.
2. By x-irradiation, cell necrosis of digital germ was appeared markedly, but in 48
hours after irradiation was depressed to the periphery of digital germ and in 72hours
after irradiation was disappeared. Digital radiating line showed marked state of
malformation in 48hours after irradiation and continued to show the same amount of
physiological cell necrosis as the compared control group in 72 hours. after irradiation.
But in the Syndactylia, physiological cell necrosis was not able to be recognized.
3. Ectrodactylia induced by x-irradiation was considered as the direct result of cell
necrosis of digital origin, however, Polydactylia and Syndactylia were considered as the
result of some effect in repair process of x-irradiation damages.